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KMID : 1094719960010010026
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Biotechnology and Bioprocess Engineering
1996 Volume.1 No. 1 p.26 ~ p.31
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Shear effects on production of lignin peroxidase byPhanerochaete chrysosporium
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Sang Byeong-In
Kim Young-Hwan
Yoo Young-Je
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Abstract
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Since biosynthesis of lignin peroxidase fromPhanerochaete chrysosporium was known to be sensitive to shear, it is interesting to understand the effects of the shear sensitivity for the over production of lignin peroxidase. In stirred-tank fermentor, the shear-sensitivity in lignin peroxidase biosynthesis was quantified by using Kolmogorov length scale. It was found that agitation at 80 μm Kolmogorov length scale is advantageous for the production of lignin peroxidase fromP. chrysosporium. To overcome the shear sensitivity in lignin peroxidase biosynthesis caused by the agitation,P. chrysosporium was immobilized on various solid carriers. The nylon-immobilizedP. chrysosporium was chosen in the present study as a way to overcome the shear sensitivity at the ranges of above 50 μm Kolmogorov length scale. The adhesion force between immobilized cell and carrier can be predicted by thermodynamic approach and used as a criteria to select an adequate carrier materials for immobilization.
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KEYWORD
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Phancrochaete chrysosporium, shear effect, lignin peroxidase, Kolmogorov lengthscale, cell immobilization
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